Rutherford B, Schenkein H A
J Immunol. 1983 Feb;130(2):874-7.
Human monocytes cultured for up to 48 hr in serum-free, chemically defined culture media released low levels of prostaglandin. C3b, C3bi, and C3c stimulated an indomethacin-sensitive, dose-responsive increase in the amount of monocyte prostaglandin released by 18 hr after treatment. Native C3 and C3d, which do not bind to monocyte receptors, failed to stimulate increased prostaglandin release. Lymphocytes, treated and untreated, produced 10(-2) to 10(-3) as much prostaglandin as the monocytes. These data support the concept that monocytes are a significant source of leukocyte prostaglandin. They also introduce an important new biologic function for the C3 fragments C3b, C3bi, and C3c.
在无血清、化学成分明确的培养基中培养长达48小时的人单核细胞释放低水平的前列腺素。C3b、C3bi和C3c刺激了单核细胞前列腺素释放量在处理后18小时出现吲哚美辛敏感的、剂量反应性增加。天然C3和C3d不与单核细胞受体结合，未能刺激前列腺素释放增加。经处理和未处理的淋巴细胞产生的前列腺素量仅为单核细胞的10(-2)至10(-3)。这些数据支持单核细胞是白细胞前列腺素重要来源的概念。它们还为C3片段C3b、C3bi和C3c引入了一项重要的新生物学功能。
