Däubener W, Fassbender B, Meuer S, Bitter-Suermann D, Hadding U
Institut für Med. Mikrobiologie, Johannes-Gutenberg-Universität, Mainz, FRG.
Eur J Immunol. 1987 Dec;17(12):1775-9. doi: 10.1002/eji.1830171215.
The effect of C3b treatment of human monocytes on secondary antigen-dependent T cell response was studied. When antigen-specific T cell blasts were cultivated together with C3b-treated monocytes the proliferative response was inhibited in a dose-dependent fashion. This suppressive effect was specific for C3b because heat-inactivated C3b or buffer alone had no influence on T cell proliferation. In part, this suppressive effect is mediated through a C3b-induced decreased expression of class II antigens on the surface of treated monocytes, but another suppressive mechanism exists because the C3b pretreatment of monocytes also led to an inhibition of the proliferative response in a class II antigen-independent T cell proliferation system. In addition to the C3b data, our finding that treatment of monocytes with C3d resulted in a lower T cell proliferation, while C3c has no effect, suggested that C3d, which could be generated from C3b in the culture, may induce the second inhibitory mechanism.
研究了用C3b处理人单核细胞对继发性抗原依赖性T细胞反应的影响。当抗原特异性T细胞母细胞与经C3b处理的单核细胞共同培养时，增殖反应呈剂量依赖性抑制。这种抑制作用对C3b具有特异性，因为热灭活的C3b或单独的缓冲液对T细胞增殖没有影响。这种抑制作用部分是通过C3b诱导处理过的单核细胞表面II类抗原表达降低介导的，但另一种抑制机制也存在，因为单核细胞的C3b预处理在II类抗原非依赖性T细胞增殖系统中也导致增殖反应受到抑制。除了C3b的数据外，我们发现用C3d处理单核细胞会导致较低的T细胞增殖，而C3c没有作用，这表明在培养中可由C3b产生的C3d可能诱导第二种抑制机制。
