Ehrlich H P, Buttle D J, Trelstad R L, Hayashi K
J Invest Dermatol. 1983 Jan;80(1):56-60. doi: 10.1111/1523-1747.ep12531078.
Normal human fibroblasts incorporated into a collagen lattice reduce the size of that lattice over a period of time. Lattice size reduction or lattice contraction is directly related to initial cell number. When equal numbers of fibroblasts derived from patients with epidermolysis bullosa dystrophica recessive, (EBdr), are used, there is delayed lattice contraction. The EBdr fibroblasts have an altered cellular shape, when compared to normal cells, in that the EBdr cells fail to flatten out and elongate, but do attach to collagen fibers like normal fibroblasts. EBdr fibroblasts maintain a rounded shape with numerous filopodia radiating from the cell periphery and such filopodia are attached to the collagen fibers of the lattice. In monolayer tissue culture on glass surfaces, EBdr fibroblasts are three times more likely to grow over neighboring fibroblasts. EBdr cell filopodia structures are attached to the cell surfaces lying beneath them, which demonstrates another condition of altered anchorage attachment of EBdr fibroblasts.
整合到胶原晶格中的正常人成纤维细胞会在一段时间内减小该晶格的大小。晶格大小的减小或晶格收缩与初始细胞数量直接相关。当使用来自隐性营养不良性大疱性表皮松解症（EBdr）患者的等量成纤维细胞时，晶格收缩会延迟。与正常细胞相比，EBdr成纤维细胞的细胞形状发生了改变，即EBdr细胞无法变平并伸长，但确实像正常成纤维细胞一样附着在胶原纤维上。EBdr成纤维细胞保持圆形，有许多丝状伪足从细胞周边辐射出来，这些丝状伪足附着在晶格的胶原纤维上。在玻璃表面的单层组织培养中，EBdr成纤维细胞生长在相邻成纤维细胞上的可能性是其三倍。EBdr细胞丝状伪足结构附着在其下方的细胞表面，这表明了EBdr成纤维细胞锚定附着改变的另一种情况。
