van Dongen W M, Moorman A F, Destrée O H
Cell Differ. 1983 May;12(5):257-64. doi: 10.1016/0045-6039(83)90021-0.
The accumulation of the maternal pool of histone H1A in Xenopus laevis oocytes was measured by the use of a semi-quantitative immunoradiographic method. This method implies the size-fractionation of total basic protein extracts from oocytes on a polyacrylamide gel, blotting of the proteins to diazotized paper and detection of histone proteins on the blots with the aid of antibodies to histone H1A and of [125I]protein A. It is shown that the maternal H1A pool that is present in fully-grown oocytes is accumulated very early in oogenesis, mainly in early vitellogenic oocytes. Stage III oocytes (Dumont, 1972) already contain approximately 80% of the final amount of H1A that is found in mature oocytes. The minimum amount of histone H1A estimated to be present in mature oocytes is 8-10 ng.
通过使用半定量免疫放射成像方法，对非洲爪蟾卵母细胞中组蛋白H1A的母源库积累情况进行了测定。该方法包括在聚丙烯酰胺凝胶上对卵母细胞的总碱性蛋白提取物进行大小分级分离，将蛋白质印迹到重氮化纸上，并借助抗组蛋白H1A抗体和[125I]蛋白A在印迹上检测组蛋白。结果表明，完全成熟的卵母细胞中存在的母源H1A库在卵子发生早期就开始积累，主要在早期卵黄生成的卵母细胞中。III期卵母细胞（Dumont，1972）已经含有成熟卵母细胞中最终H1A含量的约80%。估计成熟卵母细胞中组蛋白H1A的最小含量为8 - 10纳克。
