视黄醇可增加支持细胞含甘露糖糖蛋白的合成与分泌。

Retinol increases synthesis and secretion of Sertoli cell mannose-containing glycoproteins.

作者信息

Galdieri M, Monaco L

出版信息

Cell Biol Int Rep. 1983 Mar;7(3):219-26. doi: 10.1016/0309-1651(83)90229-1.

Abstract

Sertoli cell isolated from prepuberal rats were cultured in a chemically defined medium in the presence or in the absence or retinol. In the two experimental conditions the incorporation of 3H-mannose into cellular glycoproteins was measured. The results obtained demonstrated a 3 fold increase of mannose incorporation into cellular glycoproteins after retinol addition without any qualitative variation of their electrophoretic profile. Sertoli cell secretory activity increased of approximately 50% after retinol administration; the electrophoretic analysis showed that two of the secretory products were selectively enhanced by the vitamin addition.

摘要

从青春期前大鼠分离出的支持细胞在化学限定培养基中培养，培养基中存在或不存在视黄醇。在这两种实验条件下，测量了3H-甘露糖掺入细胞糖蛋白的情况。所获得的结果表明，添加视黄醇后，甘露糖掺入细胞糖蛋白的量增加了3倍，而其电泳图谱没有任何定性变化。给予视黄醇后，支持细胞的分泌活性增加了约50%；电泳分析表明，添加该维生素后，两种分泌产物被选择性增强。