Eskild W, Oyen O, Beebe S, Jahnsen T, Hansson V
Institute of Medical Biochemistry, University of Oslo, Norway.
Biochem Biophys Res Commun. 1988 May 16;152(3):1504-10. doi: 10.1016/s0006-291x(88)80456-x.
The levels of mRNA for cellular retinol binding protein (CRBP) were studied in primary rat Sertoli cell cultures treated with cAMP analogues and retinol. In the presence of cyclic AMP analogues a dose- and time-dependent reduction (70-90%) of the levels of mRNA for CRBP was observed. Retinol concentrations above 10 nM induced a dose- and time-dependent increase (2-3 fold) in mRNA levels for CRBP. Assuming that CRBP is important for vitamin A action, our data indicate that both cAMP and retinol itself modulate the sensitivity of the Sertoli cells for retinol.
在经环磷酸腺苷（cAMP）类似物和视黄醇处理的原代大鼠支持细胞培养物中，研究了细胞视黄醇结合蛋白（CRBP）的mRNA水平。在存在环磷酸腺苷类似物的情况下，观察到CRBP的mRNA水平呈剂量和时间依赖性降低（70 - 90%）。高于10 nM的视黄醇浓度可诱导CRBP的mRNA水平呈剂量和时间依赖性增加（2 - 3倍）。假设CRBP对维生素A的作用很重要，我们的数据表明，cAMP和视黄醇本身均可调节支持细胞对视黄醇的敏感性。
