Auger G, Blanot D, van Heijenoort J, Nadal C, Gournay M F
Eur J Biochem. 1983 Jun 15;133(2):363-9. doi: 10.1111/j.1432-1033.1983.tb07471.x.
A low molecular weight compound, which inhibits the G1-S transition in rat hepatocytes, was purified from rat trypsin-treated serum by DEAE-cellulose chromatography and high-performance liquid chromatography on three different stationary phases. This procedure led to a 34500-fold purification with a 29% yield. Inactivation of the purified material by specific enzymes showed that the inhibitor is a glycopeptide containing a peptide moiety, N-acetylneuraminic acid and galactose residues. Amino acid analyses indicated the possible existence of a pentapeptide structure. The same purification procedure was applied to the corresponding human inhibitor. Inactivation by specific enzymes showed that it is also a glycopeptide.
一种能抑制大鼠肝细胞中G1-S期转换的低分子量化合物，通过DEAE-纤维素色谱法和在三种不同固定相上的高效液相色谱法，从经大鼠胰蛋白酶处理的血清中纯化得到。该方法实现了34500倍的纯化，产率为29%。用特定酶对纯化物质进行失活处理表明，该抑制剂是一种糖肽，含有一个肽部分、N-乙酰神经氨酸和半乳糖残基。氨基酸分析表明可能存在一种五肽结构。相同的纯化程序应用于相应的人抑制剂。用特定酶进行失活处理表明它也是一种糖肽。
