Debray H, Montreuil J
Biochimie. 1978;60(8):697-702. doi: 10.1016/s0300-9084(78)80014-5.
The fractionation of glycopeptides isolated from the surface of an established line of adult rat hepatocytes, after 48 h metabolic labeling with [14C] glucose leads to the demonstration of the presence of two types of glycopeptides: i) O-glycopeptides containing N-acetylneuraminic acid, galactose and N-acetylgalactosamine, ii) N-glycopeptides with mannose, galactose, N-acetylgluosamine, fucose and N-acetylneuraminic acid. The former yield, by alkaline reductive degradation, a trisaccharide: sialyl-galactosyl-N-acetyl-galactosaminitol, a tetrasaccharide : di-sialyl-galactosyl-N-acetyl-galactosaminitol and a small amount of the disaccharide: galactosyl-N-acetyl-galactosaminitol. The latter are alkali-stable and can be divided by ion-exchange chromatography on DEAE-cellulose into i) acidic glycopeptides, not retained on a Concanavalin A-Sepharose column and belonging to the N-acetyllactosaminic type glycopedtides and ii) neutral glycopeptides which can be further fractionated on the Con-A-Sepharose column into non-retained glycopeptides belonging to the N-acetyllactosaminic type, and glycopeptides retained on the Sepharose-Con A column, containing only N-acetylglucosamine and mannose residues and related to the oligomannosidic type glycopeptides.
用[14C]葡萄糖对成年大鼠肝细胞的一个已建系细胞表面进行48小时代谢标记后，对分离得到的糖肽进行分级分离，结果表明存在两种类型的糖肽：i）含有N-乙酰神经氨酸、半乳糖和N-乙酰半乳糖胺的O-糖肽；ii）含有甘露糖、半乳糖、N-乙酰葡糖胺、岩藻糖和N-乙酰神经氨酸的N-糖肽。前者通过碱性还原降解产生一种三糖：唾液酸基-半乳糖基-N-乙酰-半乳糖胺醇、一种四糖：二唾液酸基-半乳糖基-N-乙酰-半乳糖胺醇和少量二糖：半乳糖基-N-乙酰-半乳糖胺醇。后者对碱稳定，可通过在DEAE-纤维素上进行离子交换色谱法分为：i）酸性糖肽，不保留在伴刀豆球蛋白A-琼脂糖柱上，属于N-乙酰乳糖胺型糖肽；ii）中性糖肽，可在伴刀豆球蛋白A-琼脂糖柱上进一步分级分离为属于N-乙酰乳糖胺型的未保留糖肽，以及保留在琼脂糖-伴刀豆球蛋白A柱上的糖肽，后者仅含有N-乙酰葡糖胺和甘露糖残基，与寡甘露糖型糖肽有关。
