Madon J, Zillig W
Eur J Biochem. 1983 Jun 15;133(2):471-4. doi: 10.1111/j.1432-1033.1983.tb07487.x.
A revised procedure for the purification of DNA-dependent RNA polymerase from Halobacterium halobium, including two-phase partition, yields pure, highly active and absolutely DNA-dependent enzyme. Two forms of the enzyme, one containing, the other not containing a previously not observed component, epsilon, show striking differences in activity. RNA polymerase without component epsilon has a significant activity on poly[d(A-T)] but only insignificant activity on all other templates. The enzyme containing a stoichiometric amount of component epsilon transcribes poly[d(A-T)] and native templates efficiently.
一种从嗜盐嗜盐菌中纯化依赖DNA的RNA聚合酶的改进方法，包括双相分配，可产生纯净、高活性且绝对依赖DNA的酶。该酶有两种形式，一种含有，另一种不含先前未观察到的成分ε，它们在活性上表现出显著差异。不含成分ε的RNA聚合酶对聚[d(A-T)]有显著活性，但对所有其他模板只有微不足道的活性。含有化学计量的成分ε的酶能有效地转录聚[d(A-T)]和天然模板。
