Komatsu K, Higashimori E, Uchida K, Satoh S
Exp Neurol. 1983 Jun;80(3):499-507. doi: 10.1016/0014-4886(83)90302-3.
We report that protein synthesis inhibitors exert an inhibitory effect on the trophic action of the nerve stump. The sciatic nerve innervating the extensor digitorum longus muscles of mice was cut either as close to, or as far from, the muscle as possible. Denervation changes in the muscle were evaluated using the resting membrane potential and dose-response curves obtained by plotting acetylcholine-induced contractures. Actinomycin D (2 micrograms/kg, i.p.), ethidium bromide (10 micrograms/kg, i.p.), cycloheximide (1 or 5 mg/kg, i.p.), or chloramphenicol (100 mg/kg, p.o.) administration was immediately after neurotomy and continued daily until the day preceding muscle removal. Although denervation changes occurred significantly later in muscles with a long rather than a short nerve stump, the administrated antibiotics, excluding cycloheximide, accelerated the manifestation of denervation changes in muscles with long nerve stumps without affecting those in muscles with short nerve stumps.
我们报告，蛋白质合成抑制剂对神经残端的营养作用具有抑制效应。尽可能靠近或远离小鼠趾长伸肌切断支配该肌肉的坐骨神经。使用静息膜电位以及通过绘制乙酰胆碱诱导的挛缩得到的剂量 - 反应曲线来评估肌肉中的去神经变化。在神经切断术后立即腹腔注射放线菌素D（2微克/千克）、溴化乙锭（10微克/千克）、环己酰亚胺（1或5毫克/千克）或口服氯霉素（100毫克/千克），并每日持续给药直至肌肉切除前一天。尽管去神经变化在神经残端长的肌肉中比在神经残端短的肌肉中显著延迟出现，但除环己酰亚胺外，所给予的抗生素加速了神经残端长的肌肉中去神经变化的表现，而不影响神经残端短的肌肉中的去神经变化。
