El-On J, Zehavi U, Avraham H, Greenblatt C L
Exp Parasitol. 1983 Jun;55(3):270-9. doi: 10.1016/0014-4894(83)90023-1.
A radioimmunoassay for the quantitative determination of anti-leishmanial excreted factor (EF) antibody in rabbit sera was developed. The assay, using Leishmania tropica and Leishmania donovani promastigotes EF, purified by either extraction with phenol followed by fractionation on a Sephadex G-100 column or by the dissociation of EF antibody complexes, was shown to be sensitive and reproducible. Using monospecific anti-EF antibodies, levels of as low as 0.06-0.12 micrograms/ml of anti-EF IgG could be detected. The specificity of the assay was assessed by inhibition with homologous and heterologous EF. Only minor cross-reactivity with heterologous EF was observed, and as little as 2.5 micrograms/ml of EF could be detected. Sera from kala-azar patients showed only 1.8-3.1 times more anti-EF activity, as compared with uninfected controls. No specificity was observed with sera from kala-azar patients with regard to the type of EF used. Almost the same activity was obtained with both EF from L. tropica and L. donovani. No anti-EF antibodies were detected in sera from patients with cutaneous leishmaniasis.
建立了一种用于定量测定兔血清中抗利什曼原虫分泌因子（EF）抗体的放射免疫分析方法。该分析方法使用热带利什曼原虫和杜氏利什曼原虫前鞭毛体的EF，通过苯酚提取后在葡聚糖凝胶G - 100柱上分级分离或通过EF抗体复合物解离进行纯化，结果表明该方法灵敏且可重复。使用单特异性抗EF抗体，可检测到低至0.06 - 0.12微克/毫升的抗EF IgG水平。通过同源和异源EF抑制来评估该分析方法的特异性。仅观察到与异源EF有轻微交叉反应，并且可检测到低至2.5微克/毫升的EF。与未感染对照相比，黑热病患者血清的抗EF活性仅高1.8 - 3.1倍。对于所使用的EF类型，黑热病患者血清未显示出特异性。来自热带利什曼原虫和杜氏利什曼原虫的EF几乎获得相同的活性。皮肤利什曼病患者血清中未检测到抗EF抗体。
