Vermuë N A, Nicolay K
FEBS Lett. 1983 Jun 13;156(2):293-7. doi: 10.1016/0014-5793(83)80515-8.
Smooth muscle cell energetics of taenia caeci during relaxation, spontaneous activity and maximal contraction were investigated using 31P-NMR. In relaxed muscle obtained in calcium-free medium, [ATP], [phosphocreatine] and [sugar phosphate] were 4.4 mM, 7.7 mM and 2.8 mM, respectively. There was only a small difference in the energetics of spontaneously active and maximally contracted muscles, but under both conditions substantial changes occurred as compared with relaxed muscles. The internal pH in relaxed muscle was found to be 7.05, which acidified to 6.5 during contraction. The level of sugar phosphates was found to be not a limiting factor in energetics.
使用31P-NMR研究了盲肠带在松弛、自发活动和最大收缩过程中的平滑肌细胞能量代谢。在无钙培养基中获得的松弛肌肉中，[ATP]、[磷酸肌酸]和[磷酸糖]分别为4.4 mM、7.7 mM和2.8 mM。自发活动的肌肉和最大收缩的肌肉在能量代谢方面只有微小差异，但与松弛肌肉相比，在这两种情况下都发生了显著变化。发现松弛肌肉的内部pH值为7.05，在收缩过程中酸化至6.5。发现磷酸糖的水平不是能量代谢的限制因素。
