Zöller M, Matzku S
Immunobiology. 1983 Feb;164(1):27-41. doi: 10.1016/S0171-2985(83)80015-1.
Rat natural killer (NK)/natural cytotoxic (NC) cell activity was tested in a 4-h and a 20-h 51-Cr-release assay. In the 4-h assay, NK activity was high with peritoneal cells (PC), medium with peripheral blood lymphocytes (PBL) and spleen cells and low with bone marrow (BM), thymus and lymph node (LN) cells. The differences in lytic capacity were less pronounced in a 20-h assay. This led to the suggestion that NK/NC cells may get activated upon coculture with tumor cells during the long-term assay and that the short-term assay actually reflects the activation status of NK/NC cells in different organs rather than non-identity of cells with natural cytotoxic potential. This was supported by the following observations: 1. After in-vivo or in-vitro activation of NK cells, increased reactivity was observed in a 4-h assay especially with lymphoid cells of organs with low or medium NK activity and the differences to a priori highly active organs were significantly diminished. 2. Neither by biophysical (density) nor by biochemical (surface markers) methods differences between NK versus NC cells could be substantiated. 3. Closely related patterns of cytotoxicity were observed using adherently growing or lymphoid tumor target cells.
在4小时和20小时的51-铬释放试验中检测了大鼠自然杀伤（NK）/自然细胞毒性（NC）细胞活性。在4小时试验中，腹膜细胞（PC）的NK活性高，外周血淋巴细胞（PBL）和脾细胞的NK活性中等，骨髓（BM）、胸腺和淋巴结（LN）细胞的NK活性低。在20小时试验中，溶解能力的差异不太明显。这表明在长期试验中，NK/NC细胞可能在与肿瘤细胞共培养时被激活，短期试验实际上反映了不同器官中NK/NC细胞的激活状态，而不是具有自然细胞毒性潜力的细胞的非同一性。以下观察结果支持了这一点：1. 在体内或体外激活NK细胞后，在4小时试验中观察到反应性增加，特别是在NK活性低或中等的器官的淋巴细胞中，与先天高活性器官的差异显著减小。2. 无论是通过生物物理（密度）方法还是生化（表面标志物）方法，都无法证实NK细胞与NC细胞之间的差异。3. 使用贴壁生长的或淋巴细胞性肿瘤靶细胞观察到密切相关的细胞毒性模式。
