Zöller M, Matzku S
Immunobiology. 1983 May;164(5):349-60. doi: 10.1016/S0171-2985(83)80031-X.
Cytotoxic capacity of rat macrophages (M phi) and natural killer (NK)/natural cytotoxic cells (NC) against adherent growing, solid tumor-derived target cells was evaluated, modulating the activation status of effector cells and growth conditions of target cells. Testing a panel of target cells, cytotoxicity of NK/NC and M phi was strikingly correlated so that besides of target-cell binding structures basic lysability seems to be of influence with respect to cytotoxicity rates. Varying the in vivo growth conditions of target cells altered their lysability by M phi and NK/NC cells in the sense that ascitic versus subcutaneously (sc) grown tumors were more resistant to lysis. On the other hand, in vitro culturing did not influence susceptibility for M phi, but with some tumor lines increased lysis by NK/NC cells was observed. In the rat, the activation status of M phi and NC was not age-dependent, and NK cell activity only declined slowly with age. But cytotoxic potential of M phi obviously presents a strain characteristic, different from NK/NC cell activity, only the latter two correlating in different rat strains. Experiments to augment natural cytotoxic capacity revealed that application of Corynebacterium parvum (CP) activated M phi and NK/NC cells, while sc tumor implantation only resulted in increased NK/NC cell cytotoxicity, leaving M phi unaltered.
通过调节效应细胞的激活状态和靶细胞的生长条件，评估了大鼠巨噬细胞（M phi）和自然杀伤（NK）/自然细胞毒性细胞（NC）对贴壁生长的实体瘤来源靶细胞的细胞毒性能力。在测试一组靶细胞时，NK/NC和M phi的细胞毒性显著相关，因此除了靶细胞结合结构外，基本的可裂解性似乎也对细胞毒性率有影响。改变靶细胞的体内生长条件会改变它们对M phi和NK/NC细胞的可裂解性，即腹水瘤与皮下（sc）生长的肿瘤对裂解更具抗性。另一方面，体外培养不影响M phi的敏感性，但观察到一些肿瘤细胞系被NK/NC细胞裂解的情况增加。在大鼠中，M phi和NC的激活状态不依赖于年龄，NK细胞活性仅随年龄缓慢下降。但M phi的细胞毒性潜力明显呈现出品系特征，与NK/NC细胞活性不同，只有后两者在不同大鼠品系中相关。增强自然细胞毒性能力的实验表明，应用短小棒状杆菌（CP）可激活M phi和NK/NC细胞，而皮下肿瘤植入仅导致NK/NC细胞细胞毒性增加，M phi未发生改变。
