Kubota K, Manson L A
Int J Cancer. 1983 May 15;31(5):655-60. doi: 10.1002/ijc.2910310519.
Anti-MM2 serum, which had been prepared by immunizing C3H/He mice with syngeneic MM2 mouse mammary ascites tumor, immunoprecipitated "H-2K,D-like" molecules on DBA/2 and C3H.H-2 degrees lymph node cells as well as on somatic cell hybrids between MM2 tumor and mouse L cells. Preclearing of lysates from C3H.H-2 degrees lymph node cells with anti-H-2.31 serum removed all "H-2K,D-like" molecules reactive with anti-MM2 serum, indicating that the molecules detected by anti-MM2 serum are H-2Kd antigens. The anti-H-2.31 serum detected an "H-2K,D-like structure" on the hybrid cells and absorption of the anti-H-2.31 serum with the hybrid cells deprived the serum of anti-H-2Kd reactivity. The hybrid cells could induce antibodies against the H-2Kd antigen in C3H/He mice. These results indicate that on the hybrid cells, whose parental cells were both derived from C3H mice, there is an "H-2K,D-like structure" that has the H-2Kd private specificity. Absorption of anti-MM2 serum with EL4 cells did not affect the capacity of the serum to detect the H-2Kd antigen on C3H.H-2 degrees lymph node cells, indicating that the "H-2Kd-like structure" is distinct from "H-2K,D-like structure A" which was previously reported. Nine isozymes were examined and MM2 cells, mouse L cells, and the hybrids were found to have the same isozyme markers as those of the C3H/He mouse.
抗-MM2血清是通过用同基因的MM2小鼠乳腺腹水瘤免疫C3H/He小鼠制备的，它能在DBA/2和C3H.H-2°淋巴结细胞以及MM2肿瘤与小鼠L细胞的体细胞杂种上免疫沉淀“H-2K、D样”分子。用抗-H-2.31血清预先清除C3H.H-2°淋巴结细胞的裂解物，去除了所有与抗-MM2血清反应的“H-2K、D样”分子，表明抗-MM2血清检测到的分子是H-2Kd抗原。抗-H-2.31血清在杂种细胞上检测到一种“H-2K、D样结构”，用杂种细胞吸收抗-H-2.31血清使该血清失去了抗-H-2Kd反应性。杂种细胞能在C3H/He小鼠中诱导产生针对H-2Kd抗原的抗体。这些结果表明，在亲本细胞均来自C3H小鼠的杂种细胞上，存在一种具有H-2Kd私有特异性的“H-2K、D样结构”。用EL4细胞吸收抗-MM2血清并不影响该血清检测C3H.H-2°淋巴结细胞上H-2Kd抗原的能力，表明“H-2Kd样结构”与先前报道的“H-2K、D样结构A”不同。检测了9种同工酶，发现MM2细胞、小鼠L细胞和杂种细胞具有与C3H/He小鼠相同的同工酶标记。
