Belis J A, Adlestein L B, Tarry W F
J Androl. 1983 Mar-Apr;4(2):144-9. doi: 10.1002/j.1939-4640.1983.tb00739.x.
Estradiol partially maintained the weights of the dorsolateral prostate and seminal vesicles in castrated adult male rats. One group of animals received subcutaneous injections of estradiol (0.01 mg/kg) daily, while a second group of animals received estradiol plus bromocriptine (4.0 mg/kg). Changes in weights of accessory reproductive organs were correlated with serum prolactin levels. Estradiol significantly increased serum prolactin levels while concomitant treatment with bromocriptine caused serum prolactin to remain at castrate levels. Bromocriptine slightly decreased, but did not abolish, the effect of estradiol on weights of the dorsolateral prostate and seminal vesicles. To determine if the effect of estradiol was mediated by androgen receptors in the dorsolateral prostate and seminal vesicles, a third group of animals was treated with flutamide. Flutamide, at a dosage (20 mg/kg) that abolished the effects of dihydrotestosterone on the weights of these tissues, produced no alternation of the effects of estradiol. Treatment of animals with a combination of flutamide, bromocriptine, and estradiol did not significantly alter organ weights from the results obtained with bromocriptine and estradiol. Scatchard plot analysis of the effect of estradiol treatment on cytosol binding of estradiol demonstrated increased quantities of estradiol binding in dorsolateral prostate and seminal vesicles which correlated with the changes in weights noted. Although part of the effect of estradiol on regression of male accessory reproductive organs of castrated rats may be mediated by prolactin, the principal effect appears to be a direct action on responsive tissues.
雌二醇能部分维持成年去势雄性大鼠背外侧前列腺和精囊的重量。一组动物每天接受皮下注射雌二醇（0.01毫克/千克），而另一组动物接受雌二醇加溴隐亭（4.0毫克/千克）。附属生殖器官重量的变化与血清催乳素水平相关。雌二醇显著提高血清催乳素水平，而同时使用溴隐亭可使血清催乳素维持在去势水平。溴隐亭略微降低但并未消除雌二醇对背外侧前列腺和精囊重量的影响。为了确定雌二醇的作用是否由背外侧前列腺和精囊中雄激素受体介导，第三组动物用氟他胺进行处理。氟他胺剂量为（20毫克/千克）时可消除二氢睾酮对这些组织重量的影响，但对雌二醇的作用未产生改变。用氟他胺、溴隐亭和雌二醇联合处理动物，与单独使用溴隐亭和雌二醇相比，对器官重量没有显著影响。对雌二醇处理后细胞质中雌二醇结合情况进行Scatchard作图分析表明，背外侧前列腺和精囊中雌二醇结合量增加，这与观察到的重量变化相关。尽管雌二醇对去势大鼠雄性附属生殖器官退化的部分作用可能由催乳素介导，但主要作用似乎是对反应性组织的直接作用。
