乳酸克鲁维酵母中乳糖转运的特性分析。
Characterization of lactose transport in Kluyveromyces lactis.
作者信息
Dickson R C, Barr K
出版信息
J Bacteriol. 1983 Jun;154(3):1245-51. doi: 10.1128/jb.154.3.1245-1251.1983.
Abstract
We have determined that lactose uptake in Kluyveromyces lactis is mediated by an inducible transport system. Induction, elicited by lactose or galactose, of the transporter required protein synthesis. Transport of lactose required an energy-generating system and occurred by an active process, since an intracellular lactose concentration 175 times greater than the extracellular concentration could be obtained. The Km for lactose transport was about 2.8 mM in uninduced and lactose- or galactose-induced cells. The lactose transporters in K. lactis and Escherichia coli appear to be different since they respond uniquely to inhibition by substrate analogs.
摘要
我们已经确定，乳酸克鲁维酵母中乳糖的摄取是由一种可诱导的转运系统介导的。由乳糖或半乳糖引发的转运蛋白的诱导需要蛋白质合成。乳糖的转运需要一个能量产生系统，并且是通过一个主动过程进行的，因为细胞内乳糖浓度可以达到比细胞外浓度高175倍。在未诱导以及乳糖或半乳糖诱导的细胞中，乳糖转运的米氏常数约为2.8 mM。乳酸克鲁维酵母和大肠杆菌中的乳糖转运蛋白似乎不同，因为它们对底物类似物的抑制反应独特。
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1
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2
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Mol Cell Biol. 1981 Jul;1(7):629-34. doi: 10.1128/mcb.1.7.629-634.1981.
3
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4
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6
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7
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10
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J Bacteriol. 1979 Jan;137(1):51-61. doi: 10.1128/jb.137.1.51-61.1979.
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