Zelesco P A, Graves J A
J Cell Physiol. 1983 Jul;116(1):98-102. doi: 10.1002/jcp.1041160115.
We have studied the effect of X or gamma irradiation, of one parent of a cell hybrid, on hybrid viability and chromosome segregation. The hybrid types studied were mouse--Chinese hamster (which spontaneously lose a few hamster chromosomes) and Chinese hamster--human (which spontaneously lose most of the human complement). Preirradiation of the segregated and retained cell parent resulted in highly asymmetric hybrid survival curves; survival was greatly reduced when the retained parent was irradiated, especially for hamster-human fusions. Preirradiation of the parents of mouse-hamster hybrids modified both the direction and the extent of chromosome segregation, but no consistent effect on elimination was observed for hamster-human hybrids, and reversal of the direction of loss was never observed. These results are more consistent with the hypothesis that chromosome segregation from hybrids results from an intracellular chromosome selection, than with the hypothesis that cellular selection acts on randomly generated chromosome variants.
我们研究了细胞杂种的一方亲本经X射线或γ射线照射后对杂种活力和染色体分离的影响。所研究的杂种类型为小鼠-中国仓鼠（其会自发丢失一些仓鼠染色体）和中国仓鼠-人（其会自发丢失大部分人类染色体组）。对分离并保留下来的细胞亲本进行照射前处理，会产生高度不对称的杂种存活曲线；当保留下来的亲本受到照射时，存活率会大幅降低，尤其是对于仓鼠-人杂种。对小鼠-仓鼠杂种的亲本进行照射前处理，会改变染色体分离的方向和程度，但对于仓鼠-人杂种，未观察到对染色体消除的一致影响，且从未观察到丢失方向的逆转。这些结果与杂种染色体分离源于细胞内染色体选择这一假说更为一致，而与细胞选择作用于随机产生的染色体变体这一假说不太相符。
