Fell A F, Scott H P, Gill R, Moffat A C
J Chromatogr. 1983 Mar 11;273(1):3-17. doi: 10.1016/s0378-4347(00)80920-2.
A review of progress in the field of multichannel detection in column and thin-layer chromatography is presented, together with some novel applications of a computer-based, linear photodiode array UV--visible spectrophotometer for detection in high-performance liquid chromatography (HPLC). Computer-aided methods for simultaneous monitoring of the elution profile at three wavelengths with automatic peak detection and capture of UV spectra are described. The continuous calculation of absorbance ratios during elution is discussed as an index of peak homogeneity. A novel technique for the enhancement of qualitative identification in HPLC, based on transformation of captured spectra to the second derivative or to the decadic logarithm, is proposed. These developments are exemplified by a model system of diacetylmorphine and its principal metabolites and degradation products, morphine and 6-acetylmorphine. The potential utility of three-dimensional projections of (A, lambda, t) data is discussed in the context of pharmaceutical, bioanalytical and forensic applications.
本文综述了柱色谱和薄层色谱中多通道检测领域的进展，以及基于计算机的线性光电二极管阵列紫外-可见分光光度计在高效液相色谱（HPLC）检测中的一些新应用。描述了用于在三个波长下同时监测洗脱曲线并自动进行峰检测和紫外光谱采集的计算机辅助方法。讨论了洗脱过程中吸光度比的连续计算作为峰纯度指标的情况。提出了一种基于将采集的光谱转换为二阶导数或常用对数来增强HPLC定性鉴定的新技术。以二乙酰吗啡及其主要代谢物和降解产物吗啡和6-乙酰吗啡的模型系统为例说明了这些进展。在药物、生物分析和法医应用的背景下讨论了（A，λ，t）数据三维投影的潜在用途。
