Liebich H M
J Chromatogr. 1983 Mar 11;273(1):67-75. doi: 10.1016/s0378-4347(00)80923-8.
Total 4-heptanone is the sum of a beta-oxocarboxylic acid, 2-ethyl-3-oxohexanoic acid, and its decarboxylation product, 4-heptanone. The beta-oxocarboxylic acid is found in serum and in urine, and is detected by gas chromatography--mass spectrometry in the form of its methyl ester or its O-methyloximated acid methyl ester. The ketone is detected within the profile of volatile metabolites in serum and in urine. However, the analytical procedure includes some ketone coming from in vitro decarboxylation. Total 4-heptanone is measured by gas chromatography--mass fragmentography. The method includes a quantitative transformation of the beta-oxocarboxylic acid into the ketone. A comparative study with 270 patients with diabetes mellitus, 2 healthy individuals and 143 non-diabetic hospitalized patients showed that the urinary excretion of total 4-heptanone is increased in diabetes mellitus. The mean values are 1073 micrograms per 24 h for diabetics, compared with 207 micrograms per 24 h and 246 micrograms per 24 h for healthy individuals and Non-diabetic patients, respectively. Diabetic ketoacidosis and fasting conditions decrease the total 4-heptanone.
总4-庚酮是β-氧代羧酸（2-乙基-3-氧代己酸）及其脱羧产物4-庚酮的总和。β-氧代羧酸存在于血清和尿液中，通过气相色谱-质谱法以其甲酯或O-甲基肟化酸甲酯的形式进行检测。酮在血清和尿液中的挥发性代谢产物谱中被检测到。然而，分析过程中包括一些来自体外脱羧的酮。总4-庚酮通过气相色谱-质谱碎片分析法进行测量。该方法包括将β-氧代羧酸定量转化为酮。一项对270名糖尿病患者、2名健康个体和143名非糖尿病住院患者的比较研究表明，糖尿病患者尿中总4-庚酮的排泄量增加。糖尿病患者的平均值为每24小时1073微克，而健康个体和非糖尿病患者分别为每24小时207微克和246微克。糖尿病酮症酸中毒和禁食状态会降低总4-庚酮。
