Liebich H M, Huesgen G
J Chromatogr. 1976 Nov 3;126:465-74. doi: 10.1016/s0021-9673(01)84093-0.
A quantitative method is described for determination of 4-heptanone and its precursor in urine. The method involves a single-step extraction of urine with cyclohexane and the analysis of 1 mul of the extract by gas chromatography-mass fragmentography. The detection limit is 8-10(-12)g, corresponding to 2 mug per 1000 ml of urine. The method is linear in the concentration range of 7.6-10(-9)-1.5-10(-5)g/ml and the relative standard deviations for the individual values are between 1.2 and 10%. Approximately 40 samples can be analyzed per day or 1 sample in 7-8 min. By varying the experimental conditions, the method is applicable to other ketones.
描述了一种测定尿液中4-庚酮及其前体的定量方法。该方法包括用环己烷对尿液进行一步萃取，并通过气相色谱-质谱碎片分析法对1微升萃取液进行分析。检测限为8×10⁻¹²克，相当于每1000毫升尿液中2微克。该方法在7.6×10⁻⁹ - 1.5×10⁻⁵克/毫升的浓度范围内呈线性，各个值的相对标准偏差在1.2%至10%之间。每天大约可分析40个样品，或在7 - 8分钟内分析1个样品。通过改变实验条件，该方法可适用于其他酮类。
