Serup J
J Dermatol Surg Oncol. 1983 Jul;9(7):558-61. doi: 10.1111/j.1524-4725.1983.tb00854.x.
The effect of local anesthesia with ethyl chloride freezing on biopsy size and quality for microscopy was studied postmortem. Four-mm punch biopsies were taken from the abdominal skin by different techniques: manual punch and drill (250 RPM) at 8 degrees C and 33 to 36 degrees C, ethyl chloride, and injection of lidocaine for local anesthesia. Histologic sections were assessed, and defatted dried weight (DW) was determined as an expression of biopsy size. After ethyl chloride freezing, microscopy showed more trauma (p less than 0.001) and characteristic, marginal tissue-breaks (p less than 0.01). More biopsies (p less than 0.05) were not proper for microscopy. DW and the variances showed no differences. It is concluded that local anesthesia with ethyl chloride freezing results in tissue trauma and inferior quality of the tissue specimens for microscopy.
在尸检后研究了用氯乙烷冷冻进行局部麻醉对用于显微镜检查的活检组织大小和质量的影响。通过不同技术从腹部皮肤获取4毫米的钻孔活检组织：在8摄氏度和33至36摄氏度下手动钻孔和钻取（250转/分钟）、氯乙烷、注射利多卡因进行局部麻醉。评估组织学切片，并测定脱脂干重（DW）以表示活检组织大小。氯乙烷冷冻后，显微镜检查显示有更多创伤（p<0.001）和特征性的边缘组织断裂（p<0.01）。更多的活检组织（p<0.05）不适用于显微镜检查。DW及其方差无差异。得出的结论是，用氯乙烷冷冻进行局部麻醉会导致组织创伤以及用于显微镜检查的组织标本质量较差。
