Lemanske R F, Guthman D A, Oertel H, Barr L, Kaliner M
J Immunol. 1983 Jun;130(6):2837-42.
Type I immediate hypersensitivity reactions in human and rat skin may be followed by late phase reactions (LPR). A consistent feature of both human and rat LPR is the early histologic appearance of neutrophils, which, in rats, is followed by the later appearance (8 to 24 hr) of mononuclear cells. To determine the importance of the neutrophil in the development of LPR, rats were depleted of neutrophils using parenteral injections of vinblastine sulfate (VS). VS produced a dose-dependent neutropenia, with the maximal effect on day 4. LPR that were induced with anti-IgE, isolated mast cell granules (MCG), or purified high (greater than 10,000 daltons) and low (500 to 10,000 daltons) m.w. fractions obtained from MCG were significantly abrogated in VS-treated rats. In neutropenic rats previously immunized with complete Freund's adjuvant, the intensity of inflammatory reactions produced by skin testing with purified protein derivatives was also significantly reduced. Administration of exogenous neutrophils to neutropenic animals partially reconstituted the reduced LPR. These data confirm and extend previous observations on the contribution of neutrophils to delayed hypersensitivity reactions and provide evidence that the neutrophil is critical for the development of rat cutaneous LPR as well.
人类和大鼠皮肤中的I型速发型超敏反应可能会继发迟发相反应（LPR）。人类和大鼠LPR的一个一致特征是中性粒细胞的早期组织学表现，在大鼠中，随后会出现单核细胞（8至24小时）。为了确定中性粒细胞在LPR发展中的重要性，通过肠胃外注射硫酸长春碱（VS）使大鼠的中性粒细胞减少。VS产生剂量依赖性的中性粒细胞减少，在第4天达到最大效果。用抗IgE、分离的肥大细胞颗粒（MCG）或从MCG获得的纯化高分子量（大于10,000道尔顿）和低分子量（500至10,000道尔顿）组分诱导的LPR在VS处理的大鼠中显著减轻。在先前用完全弗氏佐剂免疫的中性粒细胞减少的大鼠中，用纯化蛋白衍生物进行皮肤试验产生的炎症反应强度也显著降低。向中性粒细胞减少的动物施用外源性中性粒细胞可部分恢复降低的LPR。这些数据证实并扩展了先前关于中性粒细胞对迟发型超敏反应贡献的观察结果，并提供证据表明中性粒细胞对大鼠皮肤LPR的发展也至关重要。
