Lemanske R F, Guthman D A, Kaliner M
J Immunol. 1983 Aug;131(2):929-33.
Mast cell-dependent late phase reactions (LPR) occur in rat skin and are characterized histologically by an early (1- to 8-hr) neutrophil-rich and later (8- to 24-hr) mononuclear cell-rich infiltrate. To extend previous observations on the role of the neutrophil in the sequential histologic development of cutaneous LPR, rats were selectively depleted of circulating neutrophils using rabbit anti-rat neutrophil antibody (anti-neut), and the effects of this treatment on the histologic intensity of LPR were analyzed. In addition, the effects of neutrophil depletion on cutaneous delayed-type hypersensitivity (DTH) reactions produced by immunization with Freund's complete adjuvant followed by intradermal challenge with purified protein derivative (PPD) were assessed. Following anti-neut treatment, cutaneous DTH reactions were unaffected. In contrast, selective neutrophil depletion resulted in significant reductions in the intensity of cutaneous LPR at both 6 to 8 and 24 hr following anti-IgE or rat mast cell granule injection. Further, statistical comparison of circulating neutrophil counts with the capacity of the animal to express LPR revealed a highly significant direct correlation. These data confirm the previous observations that rat LPR are neutrophil-dependent reactions.
肥大细胞依赖性迟发相反应（LPR）发生于大鼠皮肤，其组织学特征为早期（1至8小时）富含中性粒细胞，后期（8至24小时）富含单核细胞浸润。为扩展先前关于中性粒细胞在皮肤LPR序贯组织学发展中作用的观察，使用兔抗大鼠中性粒细胞抗体（抗中性粒细胞抗体）选择性清除大鼠循环中的中性粒细胞，并分析该处理对LPR组织学强度的影响。此外，评估了中性粒细胞清除对用弗氏完全佐剂免疫后皮内注射纯化蛋白衍生物（PPD）产生的皮肤迟发型超敏反应（DTH）的影响。抗中性粒细胞抗体处理后，皮肤DTH反应未受影响。相反，选择性清除中性粒细胞导致抗IgE或注射大鼠肥大细胞颗粒后6至8小时和24小时皮肤LPR强度显著降低。此外，循环中性粒细胞计数与动物表达LPR能力的统计比较显示出高度显著的直接相关性。这些数据证实了先前的观察结果，即大鼠LPR是中性粒细胞依赖性反应。
