Rapid, efficient cloning of murine hybridoma cells in low gelation temperature agarose.
作者信息
Civin C I, Banquerigo M L
出版信息
J Immunol Methods. 1983 Jun 24;61(1):1-8. doi: 10.1016/0022-1759(83)90002-9.
PMID:6854036
Abstract
A rapid, efficient method is described for cloning of lymphocyte hybridoma cells. The method utilizes ultra-low gelation temperature agarose.
摘要
相似文献
1
Rapid, efficient cloning of murine hybridoma cells in low gelation temperature agarose.
J Immunol Methods. 1983 Jun 24;61(1):1-8. doi: 10.1016/0022-1759(83)90002-9.
2
Cloning of murine hybridoma cells in ultra-low gelation temperature agarose.在超低胶凝温度琼脂糖中克隆小鼠杂交瘤细胞。
Methods Enzymol. 1986;121:322-7. doi: 10.1016/0076-6879(86)21030-7.
3
A single-step technique for selecting and cloning hybridomas for monoclonal antibody production.一种用于选择和克隆用于单克隆抗体制备的杂交瘤的单步技术。
Methods Enzymol. 1986;121:307-22. doi: 10.1016/0076-6879(86)21029-0.
4
Bispecific monoclonal antibodies from hybrid hybridomas.来自杂交杂交瘤的双特异性单克隆抗体。
Methods Enzymol. 1986;121:210-28. doi: 10.1016/0076-6879(86)21019-8.
5
A method for freezing hybridoma clones in 96-well microculture plates.一种在96孔微量培养板中冷冻杂交瘤克隆的方法。
Methods Enzymol. 1986;121:417-22. doi: 10.1016/0076-6879(86)21040-x.
6
Production of monoclonal antibodies by agarose-entrapped hybridoma cells.琼脂糖包埋杂交瘤细胞生产单克隆抗体。
Methods Enzymol. 1986;121:352-60. doi: 10.1016/0076-6879(86)21034-4.
7
An improved in vitro immunization procedure for the production of monoclonal antibodies.一种用于生产单克隆抗体的改进的体外免疫程序。
Methods Enzymol. 1986;121:27-33. doi: 10.1016/0076-6879(86)21005-8.
8
A rapid screening technique for monoclonal antibodies with specificity for protein antigens.一种针对蛋白质抗原有特异性的单克隆抗体的快速筛选技术。
J Immunol Methods. 1983 Jun 24;61(1):9-16. doi: 10.1016/0022-1759(83)90003-0.
9
Cryopreservation of newly formed hybridomas.新形成杂交瘤的冷冻保存。
J Immunol Methods. 1983 Aug 12;62(1):69-72. doi: 10.1016/0022-1759(83)90111-4.
10
Endothelial cell growth supplements for promoting the growth of monoclonal antibody-producing hybridoma cells.用于促进产生单克隆抗体的杂交瘤细胞生长的内皮细胞生长补充剂。
Methods Enzymol. 1986;121:293-5. doi: 10.1016/0076-6879(86)21026-5.
引用本文的文献
1
Multi-site evaluation of the BD Stem Cell Enumeration Kit for CD34(+) cell enumeration on the BD FACSCanto II and BD FACSCalibur flow cytometers.使用BD FACSCanto II和BD FACSCalibur流式细胞仪，对BD干细胞计数试剂盒进行多中心评估，用于CD34(+)细胞计数。
Cytotherapy. 2014 Nov;16(11):1558-1574. doi: 10.1016/j.jcyt.2014.03.006. Epub 2014 Jun 11.
2
Apparent processing of a soybean oil body protein accompanies the onset of oil mobilization.随着油脂动员的开始，大豆油体蛋白出现明显的加工过程。
Plant Physiol. 1990 Sep;94(1):341-9. doi: 10.1104/pp.94.1.341.
3
Monoclonal antibody analysis of p53 expression in normal and transformed cells.正常细胞和转化细胞中p53表达的单克隆抗体分析。
J Virol. 1986 Aug;59(2):444-52. doi: 10.1128/JVI.59.2.444-452.1986.
Zotero 插件