一种针对蛋白质抗原有特异性的单克隆抗体的快速筛选技术。
A rapid screening technique for monoclonal antibodies with specificity for protein antigens.
作者信息
Holmberg D, Ivars F, Edlund T
出版信息
J Immunol Methods. 1983 Jun 24;61(1):9-16. doi: 10.1016/0022-1759(83)90003-0.
PMID:6406612
Abstract
A rapid technique for screening crude supernatants from antibody-producing hybridomas against protein antigen is described. The technique involves blotting of crude hybridoma supernatants to nitrocellulose filters and revealing specific binding using radiolabeled antibodies or antigen.
摘要
描述了一种针对蛋白质抗原筛选产生抗体的杂交瘤粗提上清液的快速技术。该技术包括将杂交瘤粗提上清液印迹到硝酸纤维素滤膜上，并用放射性标记的抗体或抗原揭示特异性结合。
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