Palma P A, Seifert W E, Caprioli R M, Howell R R
J Lab Clin Med. 1983 Jul;102(1):88-94.
XRF has broad applications in the quantitative analysis of trace elements in biological materials. Evaluation of its potential for the analysis of trace elements in human milk was performed. Precision of the method was demonstrated by the low (less than 3%) coefficient of variation in reproducibility of analyses performed within a given day, on different days, and by different operators. Standard curves were constructed for human milk and correlation was high (r greater than 0.98) for each of nine metals analyzed. The lower limits of determination and minimum detection limits were calculated for nine elements. Excellent correlation (r greater than 0.99) was achieved in comparison of XRF TO AA in analysis of zinc in human milk. Preliminary results of the analysis of milk zinc, copper, and iron throughout the initial 8 months of lactation disclose a decrease in trace metal concentration, most notable for zinc, during the first 60 days.
X射线荧光光谱法（XRF）在生物材料中微量元素的定量分析方面有着广泛应用。我们对其在人乳微量元素分析中的潜力进行了评估。该方法的精密度通过给定日内、不同日期以及不同操作人员进行分析的重现性变异系数低（小于3%）得以证明。为人乳构建了标准曲线，所分析的九种金属各自的相关性都很高（r大于0.98）。计算了九种元素的测定下限和最低检测限。在人乳锌分析中，XRF与原子吸收光谱法（AA）相比具有极佳的相关性（r大于0.99）。对哺乳期最初8个月内乳汁中锌、铜和铁的分析初步结果显示，在前60天内微量元素浓度下降，锌最为明显。
