采用电感耦合等离子体原子发射光谱法测定人乳小样本中的多元素。

Multielement determination in small samples of human milk by inductively coupled plasma atomic emission spectrometry.

作者信息

Silva P R, Dorea J G, Boaventura G R

机构信息

Department of Chemistry, University of Brasilia, Brazil.

出版信息

Biol Trace Elem Res. 1997 Winter;59(1-3):57-62. doi: 10.1007/BF02783230.

DOI:10.1007/BF02783230

PMID:9522047

Abstract

Inductively coupled plasma atomic emission spectroscopy (ICP-AES) was used for routine analysis of small samples of human milk. The concentrations of calcium (Ca), copper (Cu), iron (Fe), magnesium (Mg), manganese (Mn), phosphorus (P), and zinc (Zn) were determined in 203 milk samples from postpartum women at different stages of lactation after stepwise digestion in HNO3, HClO4, and H2O2 under heat. Validation of the procedure was achieved using certified reference material of bovine liver (NBS 1577) with mean recoveries of 103.5%. The concentrations of the above elements in milk matrix were comparable with previously reported values. The analytical results from breast milk will provide reference information for mineral studies of Brazilian mothers and breast-fed infants.

摘要

电感耦合等离子体原子发射光谱法（ICP - AES）用于人乳小样本的常规分析。在产后不同泌乳阶段的203份母乳样本中，通过在加热条件下用硝酸（HNO₃）、高氯酸（HClO₄）和过氧化氢（H₂O₂）逐步消解后，测定了钙（Ca）、铜（Cu）、铁（Fe）、镁（Mg）、锰（Mn）、磷（P）和锌（Zn）的浓度。使用牛肝标准参考物质（NBS 1577）对该方法进行验证，平均回收率为103.5%。母乳中上述元素的浓度与先前报道的值相当。母乳的分析结果将为巴西母亲和母乳喂养婴儿的矿物质研究提供参考信息。