Veith M C, Butterworth A E
J Exp Med. 1983 Jun 1;157(6):1828-43. doi: 10.1084/jem.157.6.1828.
Previous studies have shown that eosinophils from eosinophilic individuals differ functionally from those of normal individuals. In order to test whether agents that might induce eosinophilia could also affect eosinophil function, we have compared the capacity of culture supernatants from mononuclear cells of eosinophilic or normal individuals to enhance eosinophil activity, as reflected by an increased killing of schistosomula of Schistosoma mansoni in vitro. An enhancing activity was detected, which increased both the antibody-dependent, and to some extent the antibody-independent killing of schistosomula by eosinophils, in the absence of complement. Under similar conditions, the supernatants failed to stimulate an otherwise undetectable neutrophil-mediated killing. The activity could be removed from the assay by washing, without reversing previous eosinophil stimulation, and was not directly toxic to the schistosomula. Preliminary characterization of the activity indicated that it was relatively heat-stable at 100 degrees C for 30 min, and had an estimated molecular weight of 35,000-45,000 as judged by G-200 Sephadex fractionation. The activity was produced by a nonlymphocytic, nonspecific esterase-containing adherent mononuclear cell in the absence of either Con A or antigenic stimulation. Significant enhancing activity was detectable after 1 h of culture and continued for at least 25 h. Protein synthesis was required for its production or release. Although the activity was detectable in supernatants from both eosinophilic and normal individuals, the supernatants that demonstrated highest activity and that could be titrated out furthest were generally derived from eosinophilic individuals, suggesting that there might be some association between eosinophilia and enhanced eosinophil function.
以往的研究表明，嗜酸性粒细胞增多个体的嗜酸性粒细胞在功能上与正常个体的不同。为了测试可能诱导嗜酸性粒细胞增多的因子是否也会影响嗜酸性粒细胞的功能，我们比较了嗜酸性粒细胞增多个体或正常个体的单核细胞培养上清液增强嗜酸性粒细胞活性的能力，这通过体外曼氏血吸虫童虫杀伤增加来反映。检测到一种增强活性，在无补体的情况下，这种活性可增强嗜酸性粒细胞对童虫的抗体依赖性杀伤，并在一定程度上增强非抗体依赖性杀伤。在类似条件下，这些上清液未能刺激原本无法检测到的中性粒细胞介导的杀伤。通过洗涤可从测定中去除该活性，且不会逆转先前对嗜酸性粒细胞的刺激，并且该活性对童虫无直接毒性。对该活性的初步表征表明，在100℃下30分钟它相对耐热，通过G - 200葡聚糖凝胶分级分离判断其估计分子量为35,000 - 45,000。该活性由非淋巴细胞、含非特异性酯酶的黏附单核细胞在无刀豆球蛋白A或抗原刺激的情况下产生。培养1小时后可检测到显著的增强活性，并持续至少25小时。其产生或释放需要蛋白质合成。尽管在嗜酸性粒细胞增多个体和正常个体的上清液中均能检测到该活性，但活性最高且可滴定程度最高的上清液通常来自嗜酸性粒细胞增多个体，这表明嗜酸性粒细胞增多与嗜酸性粒细胞功能增强之间可能存在某种关联。
