Brisson A, Wade R H
J Mol Biol. 1983 May 5;166(1):21-36. doi: 10.1016/s0022-2836(83)80048-5.
The structure of the membrane protein from urothelial plasma membrane has been investigated. A three-dimensional map has been obtained at 35 A resolution from negatively stained two-dimensional arrays of membrane proteins. The lattice space group has the symmetry p6. Twelve stain-excluding areas are resolvable on projections perpendicular to the membrane plane. In the three-dimensional reconstruction these areas appear to be restricted to one side of the membrane and form protrusions that extend 50 A out of the membrane. No periodic structure is observed at the cytoplasmic side of the membrane, which suggests that the protein is not a transmembrane protein.
对尿路上皮细胞质膜的膜蛋白结构进行了研究。通过对膜蛋白的负染二维阵列进行35埃分辨率的三维成像，获得了三维图谱。晶格空间群具有p6对称性。在垂直于膜平面的投影上可分辨出十二个无染色区域。在三维重建中，这些区域似乎局限于膜的一侧，并形成从膜表面向外延伸50埃的突起。在膜的细胞质一侧未观察到周期性结构，这表明该蛋白不是跨膜蛋白。
