Shahar A, Reuveny S, Amir A, Kotler M, Mizrahi A
J Neurosci Res. 1983;9(3):339-48. doi: 10.1002/jnr.490090310.
A new approach for cultivating dissociated cerebral neurons is described. It is based on a rapid attachment of neurons to the DEAE-cellulose cylindrical MC and their subsequent interconnection to form cell-MC conglomerates. Intensive fiber growth followed by synaptogenesis and progressive myelin formation are indicative of optimal conditions of nutrition and oxygenation.
本文描述了一种培养解离脑神经元的新方法。它基于神经元快速附着于二乙氨基乙基纤维素圆柱形微载体(MC),随后它们相互连接形成细胞-MC聚集体。密集的纤维生长,随后是突触形成和渐进性髓鞘形成,表明营养和氧合的最佳条件。