Shahar A, Reuveny S, Zhang M, Espinosa de los Monteros A, de Vellis J, Shainberg A
Department of Virology, Israel Institute for Biological Research, Ness-Ziona.
Cytotechnology. 1992;9(1-3):107-15. doi: 10.1007/BF02521737.
Dispersed neuronal and muscular elements from fetal or neonatal origin, can organize and mature in culture when grown on positively charged cylindrical microcarriers (MCS), to a stage which simulate in vivo maturation. Cells arrange themselves on the MCS to form aggregates which remain floating in the nutrient medium. In such a tridimensional organization, the neuronal tissue is capable of regenerating a network of nerve fibers which establish synapse interconnections and undergo myelination. Oligodendrocytes organize on MCS in a tridimensional pattern and produce extensive myelin-like membranes. Myoblasts in MC-cultures fuse into polynucleated myotubes which become striated and contract spontaneously. Creatine kinase and acetylcholine receptor (AChR) are formed during myogenesis in similar quantities in MC-cultures and in monolayers. When both neuronal and muscle tissues are prepared from the same fetus (autologous nerve-muscle co-cultures) and are cultured on MCS, they interconnect to form neuro-muscular junctions. Cells from both tissues, exhibit better differentiation, for longer periods in MC-cultures than they do in monolayers. The floating functional entities are easy to sample and can be harvested for ultrastructural, immunocytochemical and biochemical analysis. In addition, MC-cultures can be used as a good tool for the study of acute and chronic exposures to toxicological agents, as well as for implantation into demyelinated, injured or dystrophic tissues. In this case the MCS in the implanted entities will serve as identifiable markers.
源自胎儿或新生儿的分散神经元和肌肉成分，在带正电荷的圆柱形微载体（MCS）上生长时，可在培养中组织化并成熟至模拟体内成熟的阶段。细胞在MCS上自行排列形成聚集体，这些聚集体悬浮在营养培养基中。在这种三维组织结构中，神经元组织能够再生建立突触互连并经历髓鞘形成的神经纤维网络。少突胶质细胞在MCS上以三维模式组织化并产生大量类髓鞘膜。MC培养中的成肌细胞融合形成多核肌管，这些肌管变得有横纹并自发收缩。在MC培养物和单层培养物中，肌酸激酶和乙酰胆碱受体（AChR）在肌生成过程中以相似的量形成。当神经元和肌肉组织均取自同一胎儿（自体神经 - 肌肉共培养）并在MCS上培养时，它们相互连接形成神经肌肉接头。与单层培养相比，来自两种组织的细胞在MC培养中在更长时间内表现出更好的分化。这些漂浮的功能实体易于取样，可收获用于超微结构、免疫细胞化学和生化分析。此外，MC培养可作为研究急性和慢性接触毒理学试剂的良好工具，以及用于植入脱髓鞘、受伤或营养不良的组织。在这种情况下，植入实体中的MCS将作为可识别的标记物。
