Hirota A, Fujii S, Sakai T, Kamino K
Jpn J Physiol. 1983;33(1):85-100. doi: 10.2170/jjphysiol.33.85.
The effects of temperature on spontaneous action potential activity in the 7-9 somite embryonic precontractile chick hearts were investigated using an optical method for monitoring membrane potential. A potential-sensitive merocyanine-rhodanine dye was used as an optical probe. Cooling caused a marked decrease in the frequency of occurrence of the spontaneous action potential. This slowing was associated with a decreased rate of diastolic depolarization of the pacemaker action potential. When the temperature was lowered, a decrease in the rate of rise and a prolongation of duration of the optical action signal also occurred. In addition, cooling resulted in a disordering of the rhythmicity of the action potential, and a decrease in the conduction velocity of excitation. Q10 values for such parameters were calculated: there were slight decreases in the Q10 values for the frequency and conduction velocity for 7-9 somite embryonic hearts.
采用光学方法监测膜电位，研究了温度对7-9体节胚胎期收缩前鸡心脏自发动作电位活动的影响。一种对电位敏感的部花青罗丹宁染料用作光学探针。降温导致自发动作电位的发生频率显著降低。这种减慢与起搏动作电位舒张期去极化速率降低有关。当温度降低时，光学动作信号的上升速率降低且持续时间延长。此外，降温导致动作电位节律紊乱，兴奋传导速度降低。计算了这些参数的Q10值：7-9体节胚胎心脏的频率和传导速度的Q10值略有降低。
