Ma D I, Fondacaro J D
Life Sci. 1983 Jun 20;32(25):2849-53. doi: 10.1016/0024-3205(83)90321-1.
We have examined the effects of serum from cystic fibrosis patients, healthy human volunteers and from guinea pigs on ciliary activity of guinea pig tracheal ring explants after 48 hours in culture. Sera from 9 out of 10 cystic fibrosis patients produced ciliostasis. This is a significantly greater percentage than the 7 out of 21 serum samples from healthy volunteers that produced ciliostosis. Ninety-two percent of the explants in guinea pig serum had unaltered ciliary activity, illustrating the importance of intrinsic control in the bioassay design. These results suggest that the guinea pig tracheal ring bioassay may be of value as a means of identifying the presence of the ciliotoxic factor in cystic fibrosis serum for research use but is a poor discriminator for diagnostic purposes. Modification such as rinsing the tracheal mucosa with sterile medium and a new chamber for the microscopic observation of the tissue have simplified the assay.
我们研究了囊性纤维化患者、健康人类志愿者以及豚鼠的血清对豚鼠气管环外植体培养48小时后纤毛活动的影响。10名囊性纤维化患者中有9人的血清导致纤毛运动停滞。这一比例显著高于21份健康志愿者血清样本中导致纤毛运动停滞的7份样本的比例。92%的豚鼠血清外植体纤毛活动未改变，这说明了内在对照在生物测定设计中的重要性。这些结果表明，豚鼠气管环生物测定法作为一种识别囊性纤维化血清中纤毛毒性因子存在的手段，可能对研究有价值，但作为诊断目的的鉴别方法则效果不佳。诸如用无菌培养基冲洗气管黏膜以及用于组织显微镜观察的新腔室等改进措施简化了该测定法。
