Hayashi M, Sofuni T, Ishidate M
Mutat Res. 1983 Jun;120(4):241-7. doi: 10.1016/0165-7992(83)90096-9.
Acridine Orange fluorescent staining was applied to the micronucleus test in mice and rats. Micronuclei emitted bright green fluorescence and were easily distinguished from micronucleus-like inclusions or contaminants. In rat bone-marrow cells, micronuclei with green fluorescence could be easily distinguished from granules accidentally dispersed from broken mast cells, which showed bright red fluorescence. Therefore, it is recommended that the Acridine Orange staining method be used to provide more reliable data in the micronucleus test.
吖啶橙荧光染色应用于小鼠和大鼠的微核试验。微核发出亮绿色荧光，很容易与微核样包涵体或污染物区分开来。在大鼠骨髓细胞中，绿色荧光的微核很容易与意外从破碎肥大细胞中分散出来的颗粒区分开，后者显示亮红色荧光。因此，建议使用吖啶橙染色法在微核试验中提供更可靠的数据。
