Kishi M, Horiguchi Y, Watanabe S, Hayashi M
Section of Pharmacology and Toxicology, Kanagawa Prefectural Public Health Laboratories, Yokohama, Japan.
Mutat Res. 1992 Feb-Mar;278(2-3):205-8.
The mouse peripheral blood micronucleus assay using acridine orange supravital staining was compared with the standard bone marrow assay using urethane (ethyl carbamate)-treated mice. Urethane was intraperitoneally injected to CD-1 and BDF1 mice at doses ranging from 62 to 1000 and 62 to 250 mg/kg, respectively. Peripheral blood was collected from the tail 0, 24, 48, and 72 h and bone marrow cells were smeared at 24 and 42 h after the treatment. Although the response of micronucleus induction in peripheral reticulocytes was delayed by about 24 h compared to that in bone marrow polychromatic erythrocytes, the maximum frequencies of micronucleated young erythrocytes were comparable. Therefore, the peripheral blood micronucleus assay using the acridine orange supravital staining method may provide a good alternative to the conventional bone marrow assay.
将使用吖啶橙活体染色的小鼠外周血微核试验与使用氨基甲酸乙酯（尿烷）处理小鼠的标准骨髓试验进行了比较。分别以62至1000 mg/kg和62至250 mg/kg的剂量向CD-1和BDF1小鼠腹腔注射氨基甲酸乙酯。在处理后0、24、48和72小时从尾部采集外周血，并在处理后24和42小时涂抹骨髓细胞。尽管外周网织红细胞中微核诱导的反应比骨髓多染性红细胞中的反应延迟约24小时，但微核化幼红细胞的最大频率相当。因此，使用吖啶橙活体染色法的外周血微核试验可能是传统骨髓试验的一个良好替代方法。
