Vecchini A, Binaglia L, Floridi A, Palmerini C A, Procellati G
Neurochem Res. 1983 Mar;8(3):333-40. doi: 10.1007/BF00965723.
The utilization of double-labeled CDP-choline by cultured brain cells has been studied. CDP-choline is demonstrated to be rapidly hydrolysed into CMP and choline phosphate. The fragments, or their hydrolysis products, penetrate into the cells and are utilized for lipid synthesis. At short times after the isotope administration a rapid labeling of phosphatidylcholine was detected, when cells were incubated with CDP-choline. The same was not seen when cells were incubated with labeled choline. From these observations it can be inferred that either CDP- choline can penetrate the cell membrane or that some mechanism involving CDP-choline and leading to phospholipid synthesis can work at the external surface of the plasma membranes.
已经对培养的脑细胞对双标记的胞苷二磷酸胆碱（CDP-胆碱）的利用情况进行了研究。结果表明，CDP-胆碱可迅速水解为胞苷一磷酸（CMP）和磷酸胆碱。这些片段或其水解产物可穿透细胞并用于脂质合成。在用CDP-胆碱孵育细胞后短时间内，检测到磷脂酰胆碱迅速被标记。而在用标记的胆碱孵育细胞时则未观察到同样的情况。从这些观察结果可以推断，要么CDP-胆碱能够穿透细胞膜，要么存在某种涉及CDP-胆碱并导致磷脂合成的机制可在质膜的外表面起作用。
