Gamma-crystallin-dodecyl sulfate complexes show discrete precipitation in non-ionic detergents.
作者信息
Broekhuyse R M, Kuhlmann E D
出版信息
Ophthalmic Res. 1983;15(1):2-5. doi: 10.1159/000265229.
PMID:6856246
Abstract
Gamma-crystallin-sodium dodecyl sulfate (SDS) complexes are precipitated by Triton X-100 and other non-ionic detergents. This is accomplished as discrete lines by double diffusion, radial diffusion and other immunotechniques. Many other protein-SDS complexes including those of alpha- and beta-crystallins remain soluble. Immunoglobulin G tends to precipitate under similar conditions, a reason why the use of detergents in immunoprecipitation reactions has to be carefully controlled. The phenomenon can be used for the estimation of SDS or non-ionic detergents in the presence of gamma-crystallin.
摘要
相似文献
1
Gamma-crystallin-dodecyl sulfate complexes show discrete precipitation in non-ionic detergents.
Ophthalmic Res. 1983;15(1):2-5. doi: 10.1159/000265229.
2
The action of Triton X-100 and sodium dodecyl sulphate on lipid layers. Effect on monolayers and liposomes.曲拉通X-100和十二烷基硫酸钠对脂质层的作用。对单层膜和脂质体的影响。
J Microencapsul. 1990 Apr-Jun;7(2):255-9. doi: 10.3109/02652049009021838.
3
Comparative extraction of erythrocyte EDTA-membrane proteins by some ionic and non-ionic detergents.某些离子型和非离子型去污剂对红细胞EDTA膜蛋白的比较提取
Boll Soc Ital Biol Sper. 1980 Jun 15;56(11):1103-8.
4
Two types of haemolytic activity of detergents.洗涤剂的两种溶血活性。
Biochim Biophys Acta. 1990 Aug 17;1035(2):214-7. doi: 10.1016/0304-4165(90)90119-h.
5
Method for lyophilizing brain proteolipid preparations that increases subsequent solubilization by detergents.用于冻干脑蛋白脂质制剂的方法，该方法可提高后续去污剂的增溶效果。
J Neurochem. 1982 Dec;39(6):1733-6. doi: 10.1111/j.1471-4159.1982.tb08010.x.
6
Comparative ability of various detergents to extract proteins of Borrelia burgdorferi and Borrelia hermsii.多种去污剂提取伯氏疏螺旋体和赫氏疏螺旋体蛋白质的比较能力。
Microbiologica. 1991 Oct;14(4):307-13.
7
Isolation and characterization of betaA3-crystallin associated proteinase from alpha-crystallin fraction of human lenses.从人晶状体α-晶状体蛋白组分中分离并鉴定βA3-晶状体蛋白相关蛋白酶
Mol Vis. 2008;14:1872-85. Epub 2008 Oct 20.
8
The detergent solubility properties of a malarial (Plasmodium knowlesi) variant antigen expressed on the surface of infected erythrocytes.在受感染红细胞表面表达的一种疟疾（诺氏疟原虫）变异抗原的去污剂溶解性特性。
J Cell Biochem. 1984;24(3):297-306. doi: 10.1002/jcb.240240310.
9
Sensitivity and variability of the Bradford protein assay in the presence of detergents.在存在去污剂的情况下，考马斯亮蓝蛋白质测定法的灵敏度和变异性。
Anal Biochem. 1989 May 1;178(2):263-8. doi: 10.1016/0003-2697(89)90636-2.
10
the stability in various detergents of transferrin-transferrin receptor complexes from reticulocyte plasma membranes.
Biochim Biophys Acta. 1981 Feb 18;673(1):137-46.
Zotero 插件