Hochhaus G, Rohdewald P, Möllmann H, Greschuchna D
Res Exp Med (Berl). 1983;182(1):71-8. doi: 10.1007/BF01852289.
A dexamethasone-binding protein was detected in cytosol of five normal and three neoplastic lung tissue samples. The apparent dissociation constant of the dexamethasone protein complex ranged from 5.8 to 21 nM (11.4 +/- 5.7 nM for normal tissue cytosol, 12.1 +/- 7.7 nM for the neoplastic tissue cytosol). The binding protein sedimented at 8 S in sucrose density gradient at low ionic strength and showed a high specificity for glucocorticoids. It was concluded that the dexamethasone-binding protein had the characteristics of a glucocorticoid receptor. The glucocorticoid receptor content was determined to be 12-77 fmol/mg protein (37.1 +/- 18.9 fmol/mg protein for the normal tissue cytosol, 51.9 +/- 23.9 fmol/mg protein for the neoplastic tissue cytosol).
在5份正常肺组织样本和3份肿瘤肺组织样本的胞质溶胶中检测到一种地塞米松结合蛋白。地塞米松蛋白复合物的表观解离常数范围为5.8至21 nM（正常组织胞质溶胶为11.4±5.7 nM，肿瘤组织胞质溶胶为12.1±7.7 nM）。该结合蛋白在低离子强度的蔗糖密度梯度中以8 S沉降，对糖皮质激素表现出高特异性。得出结论，地塞米松结合蛋白具有糖皮质激素受体的特征。糖皮质激素受体含量测定为12 - 77 fmol/mg蛋白质（正常组织胞质溶胶为37.1±18.9 fmol/mg蛋白质，肿瘤组织胞质溶胶为51.9±23.9 fmol/mg蛋白质）。
