Bojar H, Heim M E, Petzinna D, Staib W
Anticancer Res. 1984 May-Jun;4(3):129-32.
Twenty-four colorectal carcinomas were assayed for estrogen and progestin receptors by sucrose gradient centrifugation. Most biopsies were either completely estrogen receptor negative or displayed low titers of specific estrogen binding. Only three tumors demonstrated moderate estrogen binding activity (10-18 fmol/mg cytosol protein). In four tumors specific progestin binding exceeded 20 fmol/mg protein. Only a minor subset of the binders sedimented at 8S. Twenty-one colorectal tumors examined for the presence of glucocorticoid receptors were found to be receptor positive, without exception. Biopsies from normal colorectal mucosa displayed minute quantities of specific 8S estrogen and progestin binding, but significant titers of specific glucocorticoid binding. Our findings support the hypothesis that estrogens and progestins are unlikely to play a major role in endocrine control of colorectal neoplasms. The role of glucocorticoids in growth control of colorectal neoplasms remains to be defined.
通过蔗糖梯度离心法对24例结肠直肠癌进行雌激素和孕激素受体检测。大多数活检标本要么完全雌激素受体阴性，要么显示出低水平的特异性雌激素结合。只有3个肿瘤表现出中度雌激素结合活性（10 - 18飞摩尔/毫克胞浆蛋白）。在4个肿瘤中，特异性孕激素结合超过20飞摩尔/毫克蛋白。只有一小部分结合物在8S沉降。检测的21例结肠直肠肿瘤均无一例外发现存在糖皮质激素受体。正常结肠直肠黏膜活检标本显示出微量的特异性8S雌激素和孕激素结合，但有显著水平的特异性糖皮质激素结合。我们的研究结果支持这样的假说，即雌激素和孕激素不太可能在结肠直肠肿瘤的内分泌控制中起主要作用。糖皮质激素在结肠直肠肿瘤生长控制中的作用仍有待确定。
