Levilliers J, Weiss M C
Somatic Cell Genet. 1983 May;9(3):407-13. doi: 10.1007/BF01539147.
Crosses have been undertaken between cells of three independent clones of dedifferentiated rat hepatoma variants to investigate whether "complementation" leading to restoration of the original differentiation would occur. Hybrids were examined between ten days and two months after fusion for the presence of intracellular albumin and for their ability to proliferate in glucose-free medium where survival requires activity of the liver-specific gluconeogenic enzymes. In none of the three possible crosses involving the three variants was evidence of reexpression of hepatic functions obtained.
已在去分化大鼠肝癌变体的三个独立克隆的细胞之间进行杂交，以研究是否会发生导致原始分化恢复的“互补作用”。在融合后十天至两个月之间检查杂种细胞，看其细胞内是否存在白蛋白，以及它们在无糖培养基中增殖的能力，在无糖培养基中存活需要肝脏特异性糖异生酶的活性。在涉及这三个变体的三种可能杂交中，均未获得肝功能重新表达的证据。
