纤溶酶切割人纤维蛋白原产生的肽段对培养的兔肾细胞的影响。

Effects of peptides cleaved from human fibrinogen by plasmin on rabbit kidney cells in culture.

作者信息

Stachurska J, Janik M, Kobus M, Luczak M, Szmigielski S, Roszkowski M, Gerdin B, Saldeen T, Kopeć M

出版信息

Thromb Res. 1983 Feb 15;29(4):419-24. doi: 10.1016/0049-3848(83)90245-1.

Abstract

Low molecular weight fibrinogen degradation products (LMW-FDP) containing a mixture of dialysable peptides cleaved from human fibrinogen by plasmin are cytotoxic to an established line of rabbit kidney cells and to primary cultures of rabbit kidney cells. The presence of LMW-FDP in a concentration of 50 micrograms/ml during the cell cultivation caused a considerable release of 51Cr from prelabelled cells and inhibited 3H-thymidine and 86Rb uptake. Among three isolated peptides of established primary structure only one, 6D: Ser-Gln-Leu-Gln-Lys-Val-Pro-Pro-Glu-Trp-Lys, induced a significant effect, i.e. it enhanced 3H-thymidine incorporation. Two others, 6A: Ala-Arg-Pro-Ala-Lys and 6E: Thr-Ser-Glu-Val-Lys, did not influence the examined parameters. Hence other components of LMW-FDP must be assumed to be responsible for the cytotoxic effect on kidney cell cultures.

摘要

低分子量纤维蛋白原降解产物（LMW - FDP）是纤溶酶从人纤维蛋白原上裂解下来的可透析肽混合物，对已建立的兔肾细胞系和兔肾细胞原代培养物具有细胞毒性。在细胞培养过程中，浓度为50微克/毫升的LMW - FDP会导致预先标记的细胞大量释放51Cr，并抑制3H - 胸腺嘧啶核苷和86Rb的摄取。在三种已确定一级结构的分离肽中，只有一种，即6D：Ser - Gln - Leu - Gln - Lys - Val - Pro - Pro - Glu - Trp - Lys，产生了显著影响，即它增强了3H - 胸腺嘧啶核苷的掺入。另外两种，6A：Ala - Arg - Pro - Ala - Lys和6E：Thr - Ser - Glu - Val - Lys，对所检测的参数没有影响。因此，必须假定LMW - FDP的其他成分对肾细胞培养物的细胞毒性作用负责。