Green C R, Severs N J
Tissue Cell. 1983;15(1):17-26. doi: 10.1016/0040-8166(83)90030-7.
A simple and rapid method for the isolation of intercalated discs from a single rat heart is described. The outstanding features of this method are (i) a large quantity of starting material is not necessary, (ii) ultracentrifugation and separating gradient steps are not required, (iii) the disc fraction is relatively pure as verified by electron microscopy, and (iv) the starting tissue is rapidly homogenized into buffer, reducing possible damage to membrane structures after animal death. The fraction is rich in intercellular junctions, with gap junctions, fasciae adherentes and maculae adherentes all appearing well preserved. It should prove a useful starting base for further purification of these junctions and of sarcolemma from a specific portion of the cell surface membrane, namely the major area of structural interaction between adjacent cardiac cells.
本文描述了一种从单个大鼠心脏中分离闰盘的简单快速方法。该方法的突出特点是：（i）无需大量起始材料；（ii）不需要超速离心和分离梯度步骤；（iii）经电子显微镜验证，盘状部分相对纯净；（iv）起始组织能迅速匀浆于缓冲液中，减少动物死亡后对膜结构可能造成的损伤。该部分富含细胞间连接，缝隙连接、黏合带和黏着斑均保存良好。它应为进一步从细胞表面膜的特定部分（即相邻心肌细胞之间结构相互作用的主要区域）纯化这些连接和肌膜提供有用的起始基础。
