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蛔虫(一种线虫)肠细胞膜和细胞间连接的冷冻断裂及超薄切片研究。

A freeze fracture and thin section study of intestinal cell membranes and intercellular junctions of a nematode, Ascaris.

作者信息

Davidson L A

出版信息

Tissue Cell. 1983;15(1):27-37. doi: 10.1016/0040-8166(83)90031-9.

DOI:10.1016/0040-8166(83)90031-9
PMID:6857633
Abstract

The microvillar and lumenal plasma membrane P-face of Ascaris intestinal cells is shown to be covered by relatively large (13 nm) particles at a fairly high density (1000/microm2), while the E-face has virtually none. The P-face of the lateral cell membranes, those separating the cells, have fewer and smaller (8 nm) particles. The intestinal cells are also shown to be connected by an apical complex of smooth septate and tricellular junctions similar to those found between some insect midgut cells. A periodic layer of tannic acid staining material is found on the cytoplasmic sides of the smooth septate junction, and when the intercellular space is filled with lanthanum, smoothly curved, 10 nm wide septal walls can be seen. Below the belt of septate junctions are a large number of gap junctions. These have closely packed arrays of particles on the P-face with some particle aggregates adhering to the closely packed pit arrays on the E-face.

摘要

蛔虫肠道细胞微绒毛和管腔质膜的P面显示被相对较大(13纳米)的颗粒以相当高的密度(每平方微米1000个)覆盖,而E面几乎没有颗粒。分隔细胞的外侧细胞膜的P面颗粒较少且较小(8纳米)。还显示肠道细胞通过类似于某些昆虫中肠细胞间发现的光滑分隔连接和三细胞连接的顶端复合体相连。在光滑分隔连接的细胞质侧发现一层周期性的单宁酸染色物质,当细胞间隙充满镧时,可以看到光滑弯曲的、10纳米宽的隔壁。在分隔连接带下方有大量的间隙连接。这些间隙连接在P面上有紧密排列的颗粒阵列,一些颗粒聚集体附着在E面上紧密排列的凹坑阵列上。

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