一种将生理和药理制剂经宫颈给药至大鼠子宫的非手术技术。

A non-surgical technique for the transcervical administration of physiological and pharmacological agents into rat uteri.

作者信息

Chwalisz K, Zou J C, Jungblut P W

出版信息

Acta Endocrinol (Copenh). 1983 May;103(1):131-7. doi: 10.1530/acta.0.1030131.

DOI:10.1530/acta.0.1030131

Abstract

A technique for the instillation of solutions into the rat uterine lumen is described. The method has been tested by following the receptor depletion/replenishment cycle after oestradiol injection and by checking on the stoichiometry of hormone/receptor translocation from the cytoplasmic compartment into the nucleus. Both crude sediments from frozen uteri and nuclei purified by a novel procedure were analyzed and gave identical results. The limitations and the advantages of the technique are discussed.

摘要

本文描述了一种向大鼠子宫腔内注入溶液的技术。该方法通过跟踪雌二醇注射后的受体消耗/补充周期以及检查激素/受体从细胞质区室转运到细胞核的化学计量关系进行了测试。对来自冷冻子宫的粗沉淀物和通过一种新方法纯化的细胞核都进行了分析，结果相同。文中讨论了该技术的局限性和优点。

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A non-surgical technique for the transcervical administration of physiological and pharmacological agents into rat uteri.一种将生理和药理制剂经宫颈给药至大鼠子宫的非手术技术。

Acta Endocrinol (Copenh). 1983 May;103(1):131-7. doi: 10.1530/acta.0.1030131.

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