Halter S A, Glick A D
Ultrastruct Pathol. 1983 Jan;4(1):23-34. doi: 10.3109/01913128309140569.
Basement membrane has been studied in vitro using human mammary lobules maintained in explant culture for periods up to 4 weeks. Morphologic similarities with basal lamina in malignant tumors in vivo have been observed, such as decrease in numbers of hemidesmosomes as determined by morphometry, reduplication and multilayering of the basal lamina, and failure to produce basal lamina after migration to the surface of the explant. Light microscopy using special stains for basement membrane has shown increasing fragmentation and loss with time in culture. These findings suggest that basement membrane changes seen in malignant tumors in vivo may not be limited to malignancy, per se, but may also be the result of other conditions.
利用在体外培养长达4周的人乳腺小叶进行了基底膜的体外研究。已观察到与体内恶性肿瘤中基膜的形态学相似性，如通过形态测量确定的半桥粒数量减少、基膜的重复和多层化，以及迁移到外植体表面后未能产生基膜。使用针对基底膜的特殊染色的光学显微镜显示，随着培养时间的延长，基底膜的碎片化和损失增加。这些发现表明，体内恶性肿瘤中所见的基底膜变化可能不仅限于恶性肿瘤本身，也可能是其他情况的结果。
