Bernards R, Schrier P I, Bos J L, Van der Eb A J
Virology. 1983 May;127(1):45-53. doi: 10.1016/0042-6822(83)90369-0.
Recently we have reported that the difference in oncogenic potential between adenovirus type 5 (Ad5)-and Ad12-transformed cells in athymic nude mice is specified by early region 1b. In order to determine which of the two early region 1b (E1b) tumor antigens is responsible for the observed difference in oncogenicity we have constructed two Ad5/Ad12 hybrid plasmids: one allowing expression of the Ad5 19kD and Ad12 54kD E1b proteins, the other of the Ad5 58kD plus Ad12 19kD E1b polypeptides. Both hybrid plasmids contain the intact E1a regions of both serotypes. The chimeric plasmids were used to transform primary cultures of baby rat kidney cells and the resulting transformed cells were tested for oncogenicity in athymic nude mice. It was found that the degree of oncogenicity is determined by the identity of the large E1b tumor antigen. Studies with cells transformed by an Ad12 region E1 plasmid in which the gene coding for the 19kD tumor antigen was mutated showed that expression of this protein is nevertheless required for manifestations of the oncogenic phenotype of the transformed cell.
最近我们报道，无胸腺裸鼠中5型腺病毒（Ad5）和12型腺病毒（Ad12）转化细胞的致癌潜能差异由早期区域1b决定。为了确定两种早期区域1b（E1b）肿瘤抗原中哪一种导致了所观察到的致癌性差异，我们构建了两种Ad5/Ad12杂交质粒：一种允许表达Ad5 19kD和Ad12 54kD E1b蛋白，另一种允许表达Ad5 58kD加Ad12 19kD E1b多肽。两种杂交质粒都包含两种血清型完整的E1a区域。用这些嵌合质粒转化新生大鼠肾细胞原代培养物，并对所得转化细胞在无胸腺裸鼠中进行致癌性测试。结果发现，致癌程度由大E1b肿瘤抗原的一致性决定。对用Ad12区域E1质粒转化的细胞进行研究，其中编码19kD肿瘤抗原的基因发生了突变，结果表明，这种蛋白的表达对于转化细胞致癌表型的表现仍然是必需的。
