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[非离子洗涤剂对酶电泳条件下脱氢酶和胆碱酯酶活性研究结果的影响]

[Effect of nonionic detergents on the results of studies of dehydrogenase and cholinesterase activities under conditions of enzyme electrophoresis].

作者信息

Nadirova T Ia, Slutskiĭ I P

出版信息

Vopr Med Khim. 1983 Mar-Apr;29(2):48-51.

PMID:6858035
Abstract

Nonionic detergents of polyhydroxyethylene series were shown to catalyze the reduction of trinitrotetrazolium blue in reactions involving phenazinemethosulfate and other reducing agents. An approach of dehydrogenase fractions to the micells of nonionic detergents during enzyme gel electrophoresis led to an increase in colour intensity of individual isoenzymes and to redistribution of the isoenzyme spectrum as a result of catalytic effect of the detergents.

摘要

已表明聚羟基乙烯系列的非离子型去污剂在涉及吩嗪硫酸甲酯和其他还原剂的反应中能催化三硝基四氮唑蓝的还原。在酶凝胶电泳过程中,脱氢酶组分靠近非离子型去污剂的胶束会导致各个同工酶的颜色强度增加,并由于去污剂的催化作用而使同工酶谱重新分布。

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