Mende P, Hüther F J, Kadenbach B
FEBS Lett. 1983 Jul 25;158(2):331-4. doi: 10.1016/0014-5793(83)80607-3.
The phosphate carrier of pig heart mitochondria was solubilized with Triton X-100 and purified by chromatography on hydroxylapatite. Incubation of the phosphate carrier fraction with cardiolipin stimulated the reconstituted [32P]phosphate exchange activity in liposomes, whereas increased Triton X-100 concentrations inhibited it. The effects of cardiolipin and Triton X-100 are reversible. The activation by cardiolipin is highly specific and could not be obtained with any other applied phospholipid.
猪心脏线粒体的磷酸盐载体用Triton X-100增溶，并通过羟基磷灰石层析纯化。将磷酸盐载体组分与心磷脂一起温育可刺激脂质体中重构的[32P]磷酸盐交换活性，而增加Triton X-100的浓度则会抑制该活性。心磷脂和Triton X-100的作用是可逆的。心磷脂的激活具有高度特异性，使用任何其他应用的磷脂都无法获得这种激活效果。
