一种用于凝集素结合的乳胶凝集试验。
A latex agglutination test for lectin binding.
作者信息
Pongor S, Riedl Z
出版信息
Anal Biochem. 1983 Feb 15;129(1):51-6. doi: 10.1016/0003-2697(83)90050-7.
Blood group A + H substance purified from hog gastric mucin was fixed to polystyrene particles by physical absorption to produce a lectin-agglutinable latex. Agglutination of this latex by soybean agglutinin, concanavalin A, and wheat germ agglutinin is specifically inhibited by the respective monosaccharide haptens and is not affected by known noninhibitory sugars. The sensitivity of the assay, a function of particle loading and latex concentration, approaches that of the hemagglutination test. NaCl, NaHCO3, NH4CO3, NH4-acetate, and Tris-HCl up to a concentration of 1 M do not interfere with the test. The latex suspension is stable for several months and can be stored in freeze-dried form.
从猪胃粘蛋白中纯化得到的血型A + H物质通过物理吸附固定在聚苯乙烯颗粒上，以制备凝集素可凝集的乳胶。大豆凝集素、伴刀豆球蛋白A和麦胚凝集素对这种乳胶的凝集作用可被相应的单糖半抗原特异性抑制，且不受已知非抑制性糖类的影响。该检测方法的灵敏度取决于颗粒负载量和乳胶浓度，接近血凝试验的灵敏度。浓度高达1 M的NaCl、NaHCO₃、NH₄CO₃、NH₄ - 醋酸盐和Tris - HCl不干扰该检测。乳胶悬浮液可稳定保存数月，且可以冻干形式储存。
Zotero 插件